Journal article

Publication year: 2001

Pages: 3156-3163

Journal: Journal of Clinical Microbiology

Volume number: 39

Issue number: 9

ISSN: 0095-1137

Open access status: Green

DOI Link: https://doi.org/10.1128/JCM.39.9.3156-3163.2001

Publisher: American Society for Microbiology

Abstract: 
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P>0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises.

Harvard Citation style: Origgi, F., Klein, P., Mathes, K., Blahak, S., Marschang, R., Tucker, S., et al. (2001) Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (Spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]), Journal of Clinical Microbiology, 39(9), pp. 3156-3163. https://doi.org/10.1128/JCM.39.9.3156-3163.2001

APA Citation style: Origgi, F., Klein, P., Mathes, K., Blahak, S., Marschang, R., Tucker, S., & Jacobson, E. (2001). Enzyme-linked immunosorbent assay for detecting herpesvirus exposure in Mediterranean tortoises (Spur-thighed tortoise [Testudo graeca] and Hermann's tortoise [Testudo hermanni]). Journal of Clinical Microbiology. 39(9), 3156-3163. https://doi.org/10.1128/JCM.39.9.3156-3163.2001