Journal article

Publication year: 2001

Pages: 257-262

Journal: Biomedical Chromatography

Volume number: 15

Issue number: 4

ISSN: 0269-3879

eISSN: 1099-0801

DOI Link: https://doi.org/10.1002/bmc.67

Publisher: Wiley

Abstract: 
Free D-amino acids were ascertained in the blood serum, urine and aqueous ethanolic extracts of feces of germ-free laboratory rats and a rat made gnotobiotic (tetra-associated) with species of Streptococcus, Lactobacillus and Clostridium. D-Amino acids were also determined in the brains of two germ-free rats. For comparison, D-amino acids were also measured in the blood serum of normal rats and the blood plasma, urine and feces of normal white mice. D-Enantiomers of most protein L-amino acids were detected in all physiological samples of animals. Quantities of free D-amino acids were determined as N(O)-pentafluoropropionyl-(2)-propyl esters by enantioselective gas chromatography and mass spectrometry. Stereoisomers of the bacterial marker 2,6-diaminopimelic acid, analyzed as N-trifluoroacetyl-(2)-propyl esters, were detected in feces of the gnotobiotic but not of the germ-free rat. Copyright (C) 2001 John Wiley & Sons, Ltd.

Harvard Citation style: Brückner, H. and Schieber, A. (2001) Ascertainment of D-amino acids in germ-free, gnotobiotic and normal laboratory rats, Biomedical Chromatography, 15(4), pp. 257-262. https://doi.org/10.1002/bmc.67

APA Citation style: Brückner, H., & Schieber, A. (2001). Ascertainment of D-amino acids in germ-free, gnotobiotic and normal laboratory rats. Biomedical Chromatography. 15(4), 257-262. https://doi.org/10.1002/bmc.67