Journal article

Publication year: 2001

Pages: 166-172

Journal: Biomedical Chromatography

Volume number: 15

Issue number: 3

ISSN: 0269-3879

eISSN: 1099-0801

DOI Link: https://doi.org/10.1002/bmc.57

Publisher: Wiley

Abstract: 
Amino acid (AA) enantiomers were determined as N(O)-pentafluoropropionyl-(2)-propyl esters by chiral gas chromatography-mass spectrometry (GC-MS) in 24 h samples of the urine of three healthy volunteers and in their blood sera. In urine the largest amounts were determined for D-Ser (64-199 mu mol/day) and D-Ala (24-138 mu mol/day). In blood sera, D-Ala (2.3-4.2 mu mol/ L) and D-Ser (1.0-2.9 mu mol/L) were most abundant. Varying amounts of the D-enantiomers of Thr, Pro, Asx, Glx, Phe, Tyr, Om and Lys were also found, albeit not in all urines and sera. Further, enantiomers were quantified in urine samples of two volunteers fasting for 115 h. Quantities of renally excreted D-AAs decreased in fasting, although amounts of D-Ser (69 and 77 mu mol/L urine) as well as other D-AAs were still detectable. Time-dependent analyses of urine showed that D-AAs are continuously excreted. Copyright (C) 2001 John Wiley & Sons, Ltd.

Harvard Citation style: Bruckner, H. and Schieber, A. (2001) Determination of amino acid enantiomers in human urine and blood serum by gas chromatography-mass spectrometry, Biomedical Chromatography, 15(3), pp. 166-172. https://doi.org/10.1002/bmc.57

APA Citation style: Bruckner, H., & Schieber, A. (2001). Determination of amino acid enantiomers in human urine and blood serum by gas chromatography-mass spectrometry. Biomedical Chromatography. 15(3), 166-172. https://doi.org/10.1002/bmc.57