Journal article

Publication year: 2000

Pages: 576-582

Journal: HRC: journal of high resolution chromatography

Volume number: 23

Issue number: 10

ISSN: 0935-6304

Publisher: Wiley-VCH

Abstract: 
Quantities of D-amino acids were determined in body fluids (urine, blood plasma and blood serum, milk) of mammals (hamster, horse, bovine, sheep, pig, and dog). Amino acids were isolated using a cation exchanger and converted into their N(O)-pentafluoropropionyl (or trifluoroacetyl) amino acid 2-propyl esters. Enantiomers were separated and quantified on a Chirasil-L-Val capillary column with mass spectrometric detection using selected ion monitoring, D-Enantiomers of most protein L-amino acids were detected. Largest absolute and relative amounts in most cases were determined for D-Ser and D-Ala in urine. Stereoisomers of 2,6-diaminopimelic acid were also measured in bovine, ovine, and porcine urine. Since D-amino acids were detected in all representative classes of the major orders of Mammalia, namely Artiodactyla, Perissodactyla, Rodentia, and Carnivora, and taking reports in the literature into account, it is postulated that D-amino acids occur in all mammals.

Harvard Citation style: Brückner, H. and Schieber, A. (2000) Determination of free D-amino acids in mammalia by chiral gas chromatography-mass spectrometry, HRC-JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY, 23(10), pp. 576-582

APA Citation style: Brückner, H., & Schieber, A. (2000). Determination of free D-amino acids in mammalia by chiral gas chromatography-mass spectrometry. HRC-JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY. 23(10), 576-582.