Journal article

Publication year: 1999

Pages: 281-289

Journal: FEMS Microbiology Letters

Volume number: 175

Issue number: 2

ISSN: 0378-1097

DOI Link: https://doi.org/10.1016/S0378-1097(99)00207-4

Publisher: Oxford University Press

Abstract: 
Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following: pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single chromosome containing eight NotI fragments of 1100, 850, 365, 320, 275, 40, 30 and 20 kb in size and 11 Asci fragments of 860, 470, 410, 360, 320, 250, 110, 80, 50, 30 and 20 kb. The total genome therefore comprises 3000 +/- 50 kb, The creation of a physical and genetic map of the Listeria genome was achieved by generating NotI linking clones and their use in subsequent hybridisation analysis. Using isogenic mutants harbouring additional artificial NotI restriction sites, we were able to precisely map the positions of all currently known virulence genes on the chromosome. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Harvard Citation style: von Both, U., Otten, S., Darbouche, A., Domann, E. and Chakraborty, T. (1999) Physical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome, FEMS Microbiology Letters, 175(2), pp. 281-289. https://doi.org/10.1016/S0378-1097(99)00207-4

APA Citation style: von Both, U., Otten, S., Darbouche, A., Domann, E., & Chakraborty, T. (1999). Physical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome. FEMS Microbiology Letters. 175(2), 281-289. https://doi.org/10.1016/S0378-1097(99)00207-4