Journal article

Publication year: 1999

Pages: 157-166

Journal: Journal of Chromatography B

Volume number: 728

Issue number: 2

ISSN: 0378-4347

DOI Link: https://doi.org/10.1016/s0378-4347(99)00114-0

Publisher: Elsevier

Abstract: 
The described procedure allows quantitative, highly precise and reproducible analysis of free amino acid concentrations in single polymorphonuclear leucocytes (PMLs). This method is superior to previously described procedures with regard to sample size, PML separation, sample preparation and stability, as well as the chosen fluorescence high-performance liquid chromatography procedure, and can satisfy the high demands for ultra-sensitive and comprehensive amino acid analysis, especially for the continuous surveillance of severe diseases and organ dysfunction. (C) 1999 Elsevier Science B.V. All rights reserved.

Harvard Citation style: Mühling, J., Fuchs, M., Dehne, M., Sablotzki, A., Menges, T., Weber, B., et al. (1999) Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes. Recent developments in sample preparation and high-performance liquid chromatography, Journal of Chromatography B, 728(2), pp. 157-166. https://doi.org/10.1016/s0378-4347(99)00114-0

APA Citation style: Mühling, J., Fuchs, M., Dehne, M., Sablotzki, A., Menges, T., Weber, B., & Hempelmann, G. (1999). Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes. Recent developments in sample preparation and high-performance liquid chromatography. Journal of Chromatography B. 728(2), 157-166. https://doi.org/10.1016/s0378-4347(99)00114-0