Journal article

Efficient induction of cytotoxic CD8+ T cells against exogenous proteins:: establishment and characterization of a T cell line specific for the membrane protein ActA of Listeria monocytogenes


Authors list: Bruder, D; Darji, A; Gakamsky, DM; Chakraborty, T; Pecht, I; Wehland, J; Weiss, S

Publication year: 1998

Pages: 2630-2639

Journal: European Journal of Immunology

Volume number: 28

Issue number: 9

ISSN: 0014-2980

Open access status: Bronze

Publisher: Wiley


Abstract: 
The property of listeriolysin (LLO) to introduce soluble passenger proteins into the cytosol of antigen-presenting cells allows the induction of CD8(+) cytotoxic T cells against such antigens. To overcome the potential problem of presentation of the immunodominant epitope LL091-99 by H-2K(d), a variant LLO92A was established in which Tyr 92 was replaced by Ala. Immunization of BALB/c mice with purified LLO92A failed to stimulate cytotoxic T cells specific for either the epitope LL091-99 or for any other LLO-derived peptide. Injection of mixtures of purified LLO92A and soluble nucleoprotein (NP) of influenza virus into mice resulted in a strong cytotoxic T cell response exclusively directed against NP. The LLO92A variant was successfully used to generate, propagate and characterize a CD8(+) T cell line specific for the membrane-bound virulence factor ActA of Listeria monocytogenes. Interestingly, wildtype ActA bound to the surface of live L. monocytogenes was not presented by MHC class I molecules to the CD8(+) T cell line.



Citation Styles

Harvard Citation style: Bruder, D., Darji, A., Gakamsky, D., Chakraborty, T., Pecht, I., Wehland, J., et al. (1998) Efficient induction of cytotoxic CD8+ T cells against exogenous proteins:: establishment and characterization of a T cell line specific for the membrane protein ActA of Listeria monocytogenes , European Journal of Immunology, 28(9), pp. 2630-2639. https://doi.org/10.1002/(SICI)1521-4141(199809)28:09<2630::AID-IMMU2630>3.0.CO;2-Z

APA Citation style: Bruder, D., Darji, A., Gakamsky, D., Chakraborty, T., Pecht, I., Wehland, J., & Weiss, S. (1998). Efficient induction of cytotoxic CD8+ T cells against exogenous proteins:: establishment and characterization of a T cell line specific for the membrane protein ActA of Listeria monocytogenes . European Journal of Immunology. 28(9), 2630-2639. https://doi.org/10.1002/(SICI)1521-4141(199809)28:09<2630::AID-IMMU2630>3.0.CO;2-Z


Keywords

ANTIGEN PRESENTATION; COMPLEX CLASS-I; cytotoxic T cell; EPITOPE; HEMOLYSIN; membrane protein ActA; MHC; MOLECULE; mutant listeriolysin; soluble antigen

Last updated on 2025-10-06 at 09:18