Conference paper

Publication year: 1998

Pages: 169-173

Journal: Molecular and Cellular Endocrinology

Volume number: 140

Issue number: 1-2

ISSN: 0303-7207

DOI Link: https://doi.org/10.1016/S0303-7207(98)00046-X

Conference: Ares-Serono Foundation Workshop on Developmental Endocrinology - Roles of Genes and Growth Factors During Embryogenesis and Development of Endocrine Structures

Publisher: Elsevier

Abstract: 
There is increasing evidence that in the fetal and postnatal development of the adrenal gland, trophic and differentiating effects of ACTH are locally modulated by a species-specific pattern of growth factors. As we have shown previously in human adult adrenocortical cells (HAC) in culture, IGF-I and, even more, IGF-II enhance the steroidogenesis and ACTH responsiveness. We now examined the secretion of IGFs and their binding proteins (IGFBP) in the medium of 12 serum free primary cultures of HAC by specific RIAs and [I-125]IGF ligand blot or by immunoblot, and their long-term regulation by ACTH. HAC secrete 0.41 and 0.91 ng IGF-I and IGF-II/5 x 10(5) cells per day, respectively, and their secretion is significantly stimulated 2- and 1.6-fold, respectively? by ACTH. HAC secrete at least three IGFBPs. The 43-46 kDa and the 29 kDa proteins correspond to glycosylated and fragmented forms of IGFBP-3, and the 36 kDa protein to IGFBP-2. The most abundant protein is the 24 kDa IGFBP, with identical electrophoretic mobility to IGFBP-4. IGFBP-3, as measured by RIA, is in the range of 1 ng/day. None of the IGFBPs is significantly changed by ACTH. Thus, we have evidence for a local IGF system, and the IGF-levels are in a range compatible with a physiological auto or paracrine action on steroidogenesis. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

Harvard Citation style: l'Allemand, D., Penhoat, A., Blum, W. and Saez, J. (1998) Is there a local IGF-system in human adrenocortical cells?, Molecular and Cellular Endocrinology, 140(1-2), pp. 169-173. https://doi.org/10.1016/S0303-7207(98)00046-X

APA Citation style: l'Allemand, D., Penhoat, A., Blum, W., & Saez, J. (1998). Is there a local IGF-system in human adrenocortical cells?. Molecular and Cellular Endocrinology. 140(1-2), 169-173. https://doi.org/10.1016/S0303-7207(98)00046-X