Journal article

Publication year: 1998

Pages: 89-95

Journal: European Journal of Pharmacology

Volume number: 349

Issue number: 1

ISSN: 0014-2999

DOI Link: https://doi.org/10.1016/S0014-2999(98)00170-8

Publisher: Elsevier

Abstract: 

Basal membrane permeability of epithelial cells from the lower third and the middle of rat colonic crypts is dominated by a K+ conductance as shown by ion replacement experiments. Calyculin A, an inhibitor of protein phosphatases, induced a depolarization of these cells. The depolarization was concomitant with an inhibition of membrane current. The current inhibited by calyculin A had a reversal potential identical with the theoretical K(+ )equilibrium potential indicating that the drug inhibits a basal K+ conductance. The efficiency of calyculin A was comparable with that of other well-known K+ channel blockers such as Ba2+, tetraethylammonium or quinine. In the intact tissue, calyculin A exerted an inhibitory action on forskolin-induced anion secretion, an effect which may be explained by the decrease in the driving force for Cl- exit after inhibition of cellular K+ conductance. Together with previous results, these data suggest an inhibition of epithelial K+ conductance by phosphorylation.

Harvard Citation style: Schultheiss, G. and Diener, M. (1998) Inhibition of a K+ conductance by the phosphatase inhibitor calyculin A in rat distal colon, European Journal of Pharmacology, 349(1), pp. 89-95. https://doi.org/10.1016/S0014-2999(98)00170-8

APA Citation style: Schultheiss, G., & Diener, M. (1998). Inhibition of a K+ conductance by the phosphatase inhibitor calyculin A in rat distal colon. European Journal of Pharmacology. 349(1), 89-95. https://doi.org/10.1016/S0014-2999(98)00170-8