Journal article

Publication year: 1998

Pages: 116-122

Journal: Animal Genetics

Volume number: 29

Issue number: 2

ISSN: 0268-9146

DOI Link: https://doi.org/10.1046/j.1365-2052.1998.00295.x

Publisher: Wiley

Abstract: 
Two mitochondrial DNA molecules which represent major Ovis aries mtDNA haplogroups were cloned and comparatively sequenced to assess the degree of intraspecific variation. A total of 9623 bp that correspond to 58% of both mitochondrial genomes were determined. The control region, the Cytb, ND2, ND3, ND4L, COIII and 12 tRNA genes, including the origin of L-strand replication, were completely characterized. Partial sequence information was obtained from the 12S and 16S rRNA and an additional six protein coding and six tRNA genes. The control regions of the two mtDNAs showed a nucleotide divergence of 4.34% while coding regions differed by 0.44%. The number of sheep coding region substitutions was similar to values observed in intraspecific comparisons of mitochondrial DNAs that represent remote points in genealogical trees of mice and humans. However, replacement substitutions were only observed at similar to 30% of the rate in mice and similar to 20% of the rate in humans. Nucleotide substitutions with a potential for phenotypic effects were found in the 12S aod 16S rRNA and in the ND1 and COIII genes.

Harvard Citation style: Hiendleder, S. (1998) A low rate of replacement substitutions in two major Ovis aries mitochondrial genomes, Animal Genetics, 29(2), pp. 116-122. https://doi.org/10.1046/j.1365-2052.1998.00295.x

APA Citation style: Hiendleder, S. (1998). A low rate of replacement substitutions in two major Ovis aries mitochondrial genomes. Animal Genetics. 29(2), 116-122. https://doi.org/10.1046/j.1365-2052.1998.00295.x