Journalartikel

Jahr der Veröffentlichung: 1998

Seiten: 319-328

Zeitschrift: Journal of Chromatography B

Bandnummer: 706

Heftnummer: 2

ISSN: 0378-4347

DOI Link: https://doi.org/10.1016/s0378-4347(97)00519-7

Verlag: Elsevier

Abstract: 
Pyrazinamide, the amide of pyrazinoic acid, is one of the basic therapeutic agents currently used in combination for chemotherapy of tuberculosis. A reversed-phase high-performance liquid chromatography method based on ionic pair chromatography, was developed after solid-phase extraction of the analytes from plasma with prior addition of internal standard. The main metabolites, pyrazinoic acid, 5-hydroxypyrazinoic acid and 5-hydroxypyrazinamide, were included as well as uric acid and other purine derivatives to allow detailed study of the pharmacokinetics of the drug, especially in patients with impaired kidney function. Some interesting features of the chromatographic system giving some insight in the retention mechanism and of the solid-phase extraction are discussed in detail. (C) 1998 Elsevier Science B.V.

Harvard-Zitierstil: Kraemer, H., Feltkamp, U. and Breithaupt, H. (1998) Quantification of pyrazinamide and its metabolites in plasma by ionic-pair high-performance liquid chromatography: Implications for the separation mechanism, Journal of Chromatography B, 706(2), pp. 319-328. https://doi.org/10.1016/s0378-4347(97)00519-7

APA-Zitierstil: Kraemer, H., Feltkamp, U., & Breithaupt, H. (1998). Quantification of pyrazinamide and its metabolites in plasma by ionic-pair high-performance liquid chromatography: Implications for the separation mechanism. Journal of Chromatography B. 706(2), 319-328. https://doi.org/10.1016/s0378-4347(97)00519-7