Conference paper

Publication year: 1997

Pages: 111-115

Journal: Journal of applied botany = Angewandte Botanik

Volume number: 71

Issue number: 3-4

ISSN: 0066-1759

Conference: Meeting of the COST-Action-8.22 Work-Group-on-Somaclonal-Variation-and-Recalcitrance-to-Regeneration

Publisher: Blackwell

Abstract: 

Carrot cells (Daucus carota L.) cultured in an IAA and m-inositol containing medium were able to produce adventitious roots and somatic embryos. These differentiational processes could be suppressed by a kinetin supplement, which also increases cell proliferation.

In synchronized carrot cell suspensions (using a Fluordesoxyuridin/Thymidine system) in the plus kinetin treatment soon after thymidine application the cells passed into the S-phase whereas in the kinetin free nutrient medium the entrance into the S-phase was delayed for about 1 hour during which the cells remained in the G1-phase. During this lag period some 140 proteins are synthesized in a hierachical sequence (C-14-leucine as a tracer), which were missing in the plus kinetin cultures. It remains to be seen to which extent these additional proteins synthesized during the prolonged G1-phase contribute to the ability of the cells of this treatment to enter the S-phase later and/or the ability of these cultures to differentiate adventitious roots or somatic embryos.

Harvard Citation style: Froese, C. and Neumann, K. (1997) The influence of kinetin on the protein synthesis pattern at some stages of the cell cycle of regenerative synchronized cell suspension cultures of Daucus carota L., Journal of applied botany = Angewandte Botanik, 71(3-4), pp. 111-115

APA Citation style: Froese, C., & Neumann, K. (1997). The influence of kinetin on the protein synthesis pattern at some stages of the cell cycle of regenerative synchronized cell suspension cultures of Daucus carota L.. Journal of applied botany = Angewandte Botanik. 71(3-4), 111-115.