Journal article

Publication year: 1997

Pages: 147-153

Journal: Journal of Chromatography B

Volume number: 700

Issue number: 1-2

ISSN: 0378-4347

DOI Link: https://doi.org/10.1016/s0378-4347(97)00323-x

Publisher: Elsevier

Abstract: 
Cefotaxime, given intravenously, is currently used as a broad-spectrum antibiotic for prophylaxis of intra- and postoperative infections in ocular lens surgery. A proposed therapeutic and economic alternative is the use of orally active fluoroquinolone ofloxacine as prophylactic agent. A HPLC method was developed for determination of both antibiotics in ocular aqueous humor and plasma in order to optimize dosage for safe surpassing minimal inhibitory concentration in the humor compartment. For plasma determinations a solid-phase extraction procedure was used with ciprofloxacine as internal standard. Detection limits for direct HPLC-analysis of ocular aqueous humor was 0.08 mu g/ml for all compounds, whereas in plasma 0.31 mu g/ml could be determined after solid-phase extraction. (C) 1997 Elsevier Science B.V.

Harvard Citation style: Kraemer, H., Gehrke, R., Breithaupt, A. and Breithaupt, H. (1997) Simultaneous quantification of cefotaxime, desacetylcefotaxime, ofloxacine and ciprofloxacine in ocular aqueous humor and in plasma by high-performance liquid chromatography, Journal of Chromatography B, 700(1-2), pp. 147-153. https://doi.org/10.1016/s0378-4347(97)00323-x

APA Citation style: Kraemer, H., Gehrke, R., Breithaupt, A., & Breithaupt, H. (1997). Simultaneous quantification of cefotaxime, desacetylcefotaxime, ofloxacine and ciprofloxacine in ocular aqueous humor and in plasma by high-performance liquid chromatography. Journal of Chromatography B. 700(1-2), 147-153. https://doi.org/10.1016/s0378-4347(97)00323-x