Journal article

Publication year: 1997

Pages: 1353-1359

Journal: European Journal of Immunology

Volume number: 27

Issue number: 6

ISSN: 0014-2980

eISSN: 1521-4141

DOI Link: https://doi.org/10.1002/eji.1830270609

Publisher: Wiley

Abstract: 
Immunization of mice with mixtures of listeriolysin, a pore-forming hemolysin secreted by the pathogenic bacterium Listeria monocytogenes, together with soluble ovalbumin, nucleoprotein of influenza virus, or beta-galactosidase of Escherichia coli, resulted in strong cytotoxic CD8 T cell responses to each of the respective passenger proteins in vivo. Also, the concomitant addition of either protein with listeriolysin to target cells elicited efficient sensitization of these cells which could be attributed to the pore-forming activity of listeriolysin. This response was dependent upon a functional TAP transporter and was inhibitable by brefeldin A, indicating the transfer of the soluble proteins into the cytosol and the classical major histocompatibility (MHC) class I presentation pathway. The treatment of target cells with listeriolysin under our experimental conditions did not affect cell viability and the pores generated by listeriolysin treatment were repaired within 60 min. Introduction of soluble proteins into the MHC class I presentation pathway by listeriolysin provides a powerful system to study the cytotoxic response towards intracellular pathogens and would allow for rapid screening of potential antigens in vaccine formulations.

Harvard Citation style: Darji, A., Chakraborty, T., Wehland, J. and Weiss, S. (1997) TAP-dependent major histocompatibility complex class I presentation of soluble proteins using listeriolysin, European Journal of Immunology, 27(6), pp. 1353-1359. https://doi.org/10.1002/eji.1830270609

APA Citation style: Darji, A., Chakraborty, T., Wehland, J., & Weiss, S. (1997). TAP-dependent major histocompatibility complex class I presentation of soluble proteins using listeriolysin. European Journal of Immunology. 27(6), 1353-1359. https://doi.org/10.1002/eji.1830270609