Journalartikel

Jahr der Veröffentlichung: 1997

Seiten: 862-868

Zeitschrift: European Journal of Biochemistry

Bandnummer: 244

Heftnummer: 3

ISSN: 0014-2956

Open Access Status: Bronze

DOI Link: https://doi.org/10.1111/j.1432-1033.1997.00862.x

Verlag: Wiley: No OnlineOpen

Abstract: 
Methanobacterium thermoautotrophicum (strain Marburg), which grows autotrophically on H-2 and CO2, was found to contain 2-oxoisovalerate oxidoreductase (Vor) and indolepyruvate oxidoreductase (Ior) besides pyruvate oxidoreductase (For) and 2-oxoglutarate oxidoreductase (Kor). So far, Vor and Ior have only been detected in peptide-utilizing hyperthermophilic Archaea. The four 2-oxoacid oxidoreductases were purified and characterized with respect to their subunit composition, N-terminal amino acid sequences, and catalytic properties. For and Kor were composed of four different subunits, Vor was composed of three different subunits, and Ior of two different subunits. Comparisons of the N-terminal amino acid sequences revealed that the four enzymes are structurally related to each other and to the respective enzymes from Pyrococcus and Thermococcus sp. Vor from M. thermoautotrophicum differed from Vor from Pyrococcus furiosus in being composed of only three instead of four different subunits. Evidence is presented that in the autotrophic methanogen the four 2-oxoacid oxidoreductases have anabolic functions, Vor and Ior being involved in the biosynthesis of amino acids from fatty acids taken up from the growth medium, as shown by C-14-labelling studies.

Harvard-Zitierstil: Tersteegen, A., Linder, D., Thauer, R. and Hedderich, R. (1997) Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum, European Journal of Biochemistry, 244(3), pp. 862-868. https://doi.org/10.1111/j.1432-1033.1997.00862.x

APA-Zitierstil: Tersteegen, A., Linder, D., Thauer, R., & Hedderich, R. (1997). Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum. European Journal of Biochemistry. 244(3), 862-868. https://doi.org/10.1111/j.1432-1033.1997.00862.x