Journal article

Publication year: 1997

Pages: 151-158

Journal: Histochemistry and Cell Biology

Volume number: 107

Issue number: 2

ISSN: 0301-5564

DOI Link: https://doi.org/10.1007/s004180050099

Publisher: Springer

Abstract: 
Small intensely fluorescent (SIF) cells are paraganglionic cells derived from sympathicoblasts which may serve as interneurons, endo-/paracrine cells or arterial chemoreceptors within sympathetic ganglia. Like paraganglionic cells of other locations, e.g., carotid body glomus cells, they are responsive to hypoxia. Recent studies on glomus cells and other hypoxia-sensing cells suggested the involvement of a b(558)-type cytochrome and intracellular generation of H2O2 in the process of oxygen sensing. In the present study, we demonstrate the occurrence of the small subunit of cytochrome b(558), p22phox, in SIF cells of guinea-pig sympathetic ganglia by immunohistochemistry using two different antisera. H2O2 production was monitored in explanted intact superior cervical ganglia of 2-day-old rats by confocal laser scanning analysis of rhodamine 123 fluorescence generated due to oxidation of dihydrorhodamine 123 by H2O2. Using this technique, SIF cell clusters appeared as sites of highest H2O2 production within the ganglia. Thus, SIF cells exhibit two key features of an oxidase system generating reactive oxygen species. This may be involved in the proposed chain of events in oxygen sensing, but alternative cellular functions of this system have also to be considered.

Harvard Citation style: Kummer, W. and Acker, H. (1997) Cytochrome b558 and hydrogen peroxide production in small intensely fluorescent cells of sympathetic ganglia, Histochemistry and Cell Biology, 107(2), pp. 151-158. https://doi.org/10.1007/s004180050099

APA Citation style: Kummer, W., & Acker, H. (1997). Cytochrome b558 and hydrogen peroxide production in small intensely fluorescent cells of sympathetic ganglia. Histochemistry and Cell Biology. 107(2), 151-158. https://doi.org/10.1007/s004180050099