Journal article

Publication year: 1996

Pages: 167-173

Journal: Plant Cell Reports

Volume number: 16

Issue number: 3-4

ISSN: 0721-7714

Publisher: Springer

Abstract: 
Optimization of anther culture with regard to the induction of callus formation and direct embryogenesis was obtained for interspecific hybrids of H. annuus with H. tuberosus, H. laetiflorus, and H. resinosus by investigating six different induction media and four regeneration media. One media combination (MS-I3, MS-R3 and MS-R4) used under different culture conditions (30 degrees C/35 degrees C and different dark treatments) gave up to 92.7% embryogenic anthers with an average of 8.5 embryos per anther. However, direct embryogenesis as well as callus formation showed a strong genotypic and treatment specific reaction. From 5,600 anthers of the four investigated genotypes more than 2,000 plants could be regenerated. Regenerants were characterized by morphological traits and isozyme analyses to prove their androgenetic origin.

Harvard Citation style: Nurhidayah, T., Horn, R., Rocher, T. and Friedt, W. (1996) High regeneration rates in anther culture of interspecific sunflower hybrids, Plant Cell Reports, 16(3-4), pp. 167-173

APA Citation style: Nurhidayah, T., Horn, R., Rocher, T., & Friedt, W. (1996). High regeneration rates in anther culture of interspecific sunflower hybrids. Plant Cell Reports. 16(3-4), 167-173.