Journal article

Publication year: 1996

Pages: 582-589

Journal: Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection

Volume number: 103

Issue number: 6

ISSN: 0340-8159

Publisher: Ulmer

Abstract: 
Polyclonal antibodies (PAb) against Verticillium dahliae were obtained via immunization of hens with water-soluble proteins of a fungal isolate from oilseed rape and subsequent extraction of egg yolks. The biotinylated PAb proved to be quite efficient in a double antibody sandwich (DAS-) ELISA with a strepravidin-horseradish peroxidase assay. In a dilution of the PAb of 1 : 1000, no cross-reaction with proteins from other fungal pathogens occurred. With a dilution of 1 : 4000, a high specificity towards strains of V. dahliae from oilseed rape was observed. Isolates of V. dahliae from other host plants reacted at the most only weakly, while isolates of V. albo-atrum, V. agaricinum, V. cinnabarinum and V. lecanii exhibited no reaction above the threshold.

Harvard Citation style: Fortnagel, B. and Schlosser, E. (1996) Polyclonal antibodies against Verticillium dahliae Kleb from oil-seed rape via immunized hens, Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection, 103(6), pp. 582-589

APA Citation style: Fortnagel, B., & Schlosser, E. (1996). Polyclonal antibodies against Verticillium dahliae Kleb from oil-seed rape via immunized hens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection. 103(6), 582-589.