Journalartikel

Jahr der Veröffentlichung: 1996

Seiten: 153-164

Zeitschrift: Veterinary Microbiology

Bandnummer: 52

Heftnummer: 1-2

ISSN: 0378-1135

DOI Link: https://doi.org/10.1016/0378-1135(96)00058-2

Verlag: Elsevier

Abstract: 
Naturally occurring enterohemolysin negative variants were observed during studies on bovine Shiga-like toxin-producing E. coli (SLTEC). Examination of three strains (413/89-1 and 332, O26:H-, and 570/89, O111:H-) and their isogenic variants (413/89-6, 332-I and 570/89-I, respectively) showed, that in each strain loss of the enterohemolytic phenotype correlated with the loss of a large plasmid ranging from 94 to 104 kb in size. The hemolysin determinant present on the 94 kb plasmid of strain 413/89-1 was cloned and discovered by DNA and N-terminal aminoacid sequence analysis to be highly homologous to the recently published EHEC-hemolysin (Hly(EHEC); Schmidt et al., 1994; 1995). When a recombinant plasmid harboring this determinant was reintroduced into the enterohemolysin negative isogenic mutant 413/89-6, the enterohemolytic phenotype was restored. Southern blot hybridization analysis was used to demonstrate that the Hly(EHEC) is plasmid-borne in SLTEC-strains. Our cumulative data suggest that the enterohemolytic phenotype of SLTEC is encoded by the plasmid-borne Hly(EHEC). These results further demonstrate the close similarity between SLTEC-isolates from bovine and human.

Harvard-Zitierstil: Wieler, L., Tigges, M., Ebel, F., Schaferkordt, S., Djafari, S., Schlapp, T., et al. (1996) The enterohemolysin phenotype of bovine Shiga-like toxin-producing Escherichia coli (SLTEC) is encoded by the EHEC-hemolysin gene, Veterinary Microbiology, 52(1-2), pp. 153-164. https://doi.org/10.1016/0378-1135(96)00058-2

APA-Zitierstil: Wieler, L., Tigges, M., Ebel, F., Schaferkordt, S., Djafari, S., Schlapp, T., Baljer, G., & Chakraborty, T. (1996). The enterohemolysin phenotype of bovine Shiga-like toxin-producing Escherichia coli (SLTEC) is encoded by the EHEC-hemolysin gene. Veterinary Microbiology. 52(1-2), 153-164. https://doi.org/10.1016/0378-1135(96)00058-2