Journal article

Publication year: 1996

Pages: 311-318

Journal: Japanese journal of physiology

Volume number: 46

Issue number: 4

ISSN: 0021-521X

DOI Link: https://doi.org/10.2170/jjphysiol.46.311

Publisher: Physiological Society of Japan

Abstract: 
Properties of hypotonically-activated Cl- channels in isolated rat hepatocytes were studied by the patch-clamp whole-cell technique. Hypotonic stress (140-150 mosmol kg(-1) H2O) induced a hyperpolarization of the membrane of hepatocytes in the presence of an inwardly oriented Cl- gradient, but had no effect on the membrane potential in the absence of Cl-. An increase in the hypotonically-induced conductance was significantly inhibited by 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS; 50 mu M), but not by Ba2+ (1 mM). Pre-incubation with arachidonic acid (20 mu M) significantly inhibited the hypotonically-activated conductance. The combined application of a cyclo-oxygenase inhibitor, indomethacin (50 mu M) and a lipoxygenase inhibitor, esculetin (100 mu M) also inhibited the conductance, whereas quinacrine (200 mu M), a phospholipase A(2) inhibitor, significantly induced a large steady conductance. Outward Cl- currents, but not cationic currents, were elicited by the hypotonic stress. The current did not show any rapid time-dependent inactivation during the voltage clamp of 0.1 s. The combined application of arachidonic acid, indomethacin and esculetin inhibited the hypotonically-activated Cl- currents. The present study has shown that the Cl- channel is activated by exposure to hypotonic stress and closed by an increase in arachidonic acid concentration in isolated rat hepatocytes.

Harvard Citation style: Sakai, H., Kakinoki, B., Diener, M. and Takeguchi, N. (1996) Endogenous arachidonic acid inhibits hypotonically-activated Cl- channels in isolated rat hepatocytes, Japanese journal of physiology, 46(4), pp. 311-318. https://doi.org/10.2170/jjphysiol.46.311

APA Citation style: Sakai, H., Kakinoki, B., Diener, M., & Takeguchi, N. (1996). Endogenous arachidonic acid inhibits hypotonically-activated Cl- channels in isolated rat hepatocytes. Japanese journal of physiology. 46(4), 311-318. https://doi.org/10.2170/jjphysiol.46.311