Journal article

Publication year: 1996

Pages: 453-458

Journal: Histochemistry and Cell Biology

Volume number: 105

Issue number: 6

ISSN: 0301-5564

DOI Link: https://doi.org/10.1007/BF01457658

Publisher: Springer

Abstract: 
Sensory ganglia (trigeminal, jugular, nodose, cervical and lumbar dorsal root ganglia) of the guineapig were investigated fbr the presence of a constitutive carbon monoxide-generating enzyme, heme oxygenase-2 (HO-2). A 36-kDa HO-2-immunoreactive protein was identified by Western blotting in protein extracts from dorsal root ganglia and localized by immunohistochemistry to all neuronal perikarya, including both substance P-positive and substance P-negative neurons, in all ganglia investigated. This ubiquitous distribution points to a general requirement for HO-2 in primary afferent neurons rather than to an association with a specific functionally defined subpopulation. Neither the axons of the sensory neurons nor their peripheral terminals in the skin and around visceral arteries were HO-2 immunoreactive. Explants of dorsal root ganglia with crushes placed on the dorsal, roots showed accumulation of the neuropeptide, substance P, at the ganglionic side of the crush, but these axons were non-reactive to HO-2, indicating that there is no substantial transport of HO-2 towards the central ending of these sensory neurons. Collectively, the findings suggest that HO-2 exerts it major effects within the sensory ganglia themselves.

Harvard Citation style: Vollerthun, R., Hohler, B. and Kummer, W. (1996) Heme oxygenase-2 in primary afferent neurons of the guinea-pig, Histochemistry and Cell Biology, 105(6), pp. 453-458. https://doi.org/10.1007/BF01457658

APA Citation style: Vollerthun, R., Hohler, B., & Kummer, W. (1996). Heme oxygenase-2 in primary afferent neurons of the guinea-pig. Histochemistry and Cell Biology. 105(6), 453-458. https://doi.org/10.1007/BF01457658