Journal article

Publication year: 1996

Pages: 75-81

Journal: International Journal of Antimicrobial Agents

Volume number: 7

Issue number: 1

ISSN: 0924-8579

DOI Link: https://doi.org/10.1016/0924-8579(96)00014-3

Publisher: Elsevier

Abstract: 
In the present study we tested the ability of beta-lactam antibiotics and other cell-wall antibiotics to inhibit the production of listeriolysin which is the main virulence factor of L. monocytogenes. The amount of listeriolysin produced was measured in the supernatants with a sensitive hemolysin assay and as beta-galactosidase expression from the listeriolysin promoter. When tested in concentrations that did not affect growth of the bacteria, all beta-lactam antibiotics and all other cell-wall antibiotics tested were able to reduce the listeriolysin production in at least one of the tests used. Yet, no change in beta-galactosidase activity was detected in a strain harboring a control beta-galactosidase fusion. We therefore demonstrate for the first time that subinhibitory concentrations of antibiotics Specifically reduce the production of a virulence factor of L. monocytogenes.

Harvard Citation style: Nichterlein, T., Domann, E., Kretschmar, M., Bauer, M., Hlawatsch, A., Hof, H., et al. (1996) Subinhibitory concentrations of beta-lactams and other cell-wall antibiotics inhibit listeriolysin production by Listeria monocytogenes, International Journal of Antimicrobial Agents, 7(1), pp. 75-81. https://doi.org/10.1016/0924-8579(96)00014-3

APA Citation style: Nichterlein, T., Domann, E., Kretschmar, M., Bauer, M., Hlawatsch, A., Hof, H., & Chakraborty, T. (1996). Subinhibitory concentrations of beta-lactams and other cell-wall antibiotics inhibit listeriolysin production by Listeria monocytogenes. International Journal of Antimicrobial Agents. 7(1), 75-81. https://doi.org/10.1016/0924-8579(96)00014-3