Journal article

Publication year: 1996

Pages: 1002-1006

Journal: Infection and Immunity

Volume number: 64

Issue number: 3

ISSN: 0019-9567

eISSN: 1098-5522

Open access status: Green

DOI Link: https://doi.org/10.1128/IAI.64.3.1002-1006.1996

Publisher: American Society for Microbiology

Abstract: 
Monoclonal antibodies were generated against a 30-kDa protein fraction derived from culture supernatants of a Listeria monocytogenes strain complemented with additional copies of the prfA regulator gene, Several of the antibodies reacted specifically with a hitherto unidentified, secreted 30-kDa polypeptide, By immunoblot analysis, the expression of this 30-kDa polypeptide was found to be dependent on the presence of the PrfA regulator protein, Microsequencing of peptides derived from the partially purified 30 kBa protein revealed homologies to the InlA and InlB polypeptides of L. monocytogenes, which are required for the internalization. of the bacteria into nonphagocytic cell lines, This prompted us to term the 30-kDa polypeptide internalin-related protein (Irp). Irp-specific monoclonal antibodies cross-reacted with a 24-kDa polypeptide present in culture supernatants of Listeria ivanovii, indicating the existence of an Irp-related protein In this pathogenic Listeria species.

Harvard Citation style: Lingnau, A., Chakraborty, T., Niebuhr, K., Domann, E. and Wehland, J. (1996) Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii, Infection and Immunity, 64(3), pp. 1002-1006. https://doi.org/10.1128/IAI.64.3.1002-1006.1996

APA Citation style: Lingnau, A., Chakraborty, T., Niebuhr, K., Domann, E., & Wehland, J. (1996). Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii. Infection and Immunity. 64(3), 1002-1006. https://doi.org/10.1128/IAI.64.3.1002-1006.1996