Journal article
Authors list: Vaupel, M; Dietz, H; Linder, D; Thauer, RK
Publication year: 1996
Pages: 294-300
Journal: European Journal of Biochemistry
Volume number: 236
Issue number: 1
ISSN: 0014-2956
Open access status: Bronze
DOI Link: https://doi.org/10.1111/j.1432-1033.1996.00294.x
Publisher: Wiley: No OnlineOpen
Abstract:
The gene mch encoding N-5,N-10-methenyltetrahydromethanopterin cyclohydrolase (Mch) in Methanobacterium thermoautotrophicum (strain Marburg) was cloned and sequenced. The gene, 963 bp, was found to be located at the 3' end of a 3.5-kbp BamHI fragment. Upstream of the mch gene two open reading frames were recognized, one encoding for a 25-kDa protein with sequence similarity to deoxyuridylate hydroxymethylase and the other encoding for a 34.6-kDa protein with sequence similarity to cobalamin-independent methionine synthase (MetE). The N-terminal amino acid sequence deduced for the deoxyuridylate hydroxymethylase was identical to that previously published for thymidylate synthase (TysY) from M. thermoautotrophicum. The 3' end of the tysY gene overlapped by 8 bp with the 5' end of the mch gene. Despite this fact, the mch gene appeared to be transcribed monocistronically as evidenced by Northern blot analysis and primer-extension experiments. The mch gene was overexpressed in Escherichia coli yielding an active enzyme of 37 kDa with a specific activity of 30 U/mg cell extract protein.
Citation Styles
Harvard Citation style: Vaupel, M., Dietz, H., Linder, D. and Thauer, R. (1996) Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli, European Journal of Biochemistry, 236(1), pp. 294-300. https://doi.org/10.1111/j.1432-1033.1996.00294.x
APA Citation style: Vaupel, M., Dietz, H., Linder, D., & Thauer, R. (1996). Primary structure of cyclohydrolase (Mch) from Methanobacterium thermoautotrophicum (strain Marburg) and functional expression of the mch gene in Escherichia coli. European Journal of Biochemistry. 236(1), 294-300. https://doi.org/10.1111/j.1432-1033.1996.00294.x
Keywords
5,10-METHENYLTETRAHYDROMETHANOPTERIN CYCLOHYDROLASE; DELTA-H; FORMYLMETHANOFURAN; methenyltetrahydromethanopterin cyclohydrolase; methionine synthase; serine hydroxymethylase; TETRAHYDROMETHANOPTERIN FORMYLTRANSFERASE; THERMOPHILE METHANOPYRUS-KANDLERI; thymidylate synthase
