Journalartikel

Jahr der Veröffentlichung: 1996

Seiten: 83-93

Zeitschrift: Biotechnology and Applied Biochemistry

Bandnummer: 23

ISSN: 0885-4513

Verlag: Wiley

Abstract: 
We have analysed the status and transcriptional activity of the bovine papillomavirus-1 (complete BPV-1 genome)-based expression vector pCES in C127i-cell-line-derived 3T1 cells used for the industrial production of recombinant human erythropoietin (rhuEpo). Complete tandem head-to-tail integration of about 600 vector copies at a single site of the cellular genome was observed. Deletions, insertions or rearrangements of pCES-specific sequences or extrachromosomal copies of vector sequences were not detected. Transcriptional analyses demonstrated that rhuEpo was abundantly expressed, BPV-1 early transcription was detected, as expected from a BPV-1-transformed cell line; however, compared with the mouse metallothionein-1-promoter-driven rhuEpo-specific transcription, it was very weak. Late BPV-1 transcription was also not detected in 3T1 cells under conditions of large-scale rhuEpo production. Therefore this expression system proved to be safe and suitable for the production of rhuEpo.

Harvard-Zitierstil: Fibi, M., Kunz, J., Weimer, T., Schulz, R., Teifke, P., Grzeschik, K., et al. (1996) Status and transcriptional activity of a bovine-papillomavirus-I-based expression vector in a recombinant production cell line, Biotechnology and Applied Biochemistry, 23, pp. 83-93

APA-Zitierstil: Fibi, M., Kunz, J., Weimer, T., Schulz, R., Teifke, P., Grzeschik, K., Johannsen, R., & Zettlemeissl, G. (1996). Status and transcriptional activity of a bovine-papillomavirus-I-based expression vector in a recombinant production cell line. Biotechnology and Applied Biochemistry. 23, 83-93.