Journal article

Publication year: 1996

Pages: 96-102

Journal: European Urology

Volume number: 30

Issue number: 1

ISSN: 0302-2838

eISSN: 1873-7560

Publisher: Elsevier

Abstract: 
Objective: to evaluate the significance of HPV type 6b, 11, 16 and 18 together with type-specific antibodies in the serum of bladder carcinoma. Methods: The prevalence of HPV type 6b, 11, 16 and 18 in bladder tumor, normal bladder and urethra together with type-specific antibodies in serum was investigated in 23 patients with bladder cancer and 9 patients with chronic cystitis. HPV DNA analysis was done by polymerase chain reaction (PCR). Open reading frames of HPV were expressed in Escherichia coli as beta-galactosidase fusion proteins. Results: HPV 6b was demonstrated in the tumor tissue of 6 patients (19%), and in the nonmalignant specimens of 6 further patients (19%). HPV 16/18 was only found in the urethral swabs of 2 patients (6%). Anti-HPV antibodies were positive in 7 patients (22%). There was no association between the demonstration of HPV 6b and the occurrence of bladder tumor in this study. Conclusion: Though, in this study, HPV was not associated with bladder cancer, further investigation is necessary to elucidate the role of HPV 6b in bladder tissue possibly by a semiquantitative PCR in tissue samples and of anti-HPV antibodies in serum.

Harvard Citation style: Ludwig, M., Kochel, H., Fischer, C., Ringert, R. and Weidner, W. (1996) Human papillomavirus in tissue of bladder and bladder carcinoma specimens - A preliminary study, European Urology, 30(1), pp. 96-102

APA Citation style: Ludwig, M., Kochel, H., Fischer, C., Ringert, R., & Weidner, W. (1996). Human papillomavirus in tissue of bladder and bladder carcinoma specimens - A preliminary study. European Urology. 30(1), 96-102.