Journalartikel

Jahr der Veröffentlichung: 1996

Seiten: 103-108

Zeitschrift: Folia Histochemica et Cytobiologica

Bandnummer: 34

Heftnummer: 2

ISSN: 0239-8508

eISSN: 1897-5631

Verlag: Polskiego Towarzystwa Histochemików i Cytochemików (Polish Histochemical and Cytochemical Society) / Via Medica

Abstract: 
Cells of the zygnematophycean green alga Mougeotia contain numerous globules with polyphenolic matrix, which resemble physodes. In order to analyse the capability of this compartment to sequester various ions, trichomes of Mougeotia scalaris were either fixed for X-ray microanalysis simultaneously in 2% glutardialdehyde/1% OsO4 in phosphate buffers of different K+/Na+-ratios, or embedded directly (fresh material) in Nanoplast(R) resin. In addition, fixed material was treated with potassium antimonate and Ca2+ localization was examined by electron microscopic cytochemistry. A Ca2+-depletion upon fixation at different K+/Na+-ratios resulted in selective uptake of potassium, but not sodium. Consistent with earlier findings. calcium-binding by the polyphenolic physode matrix does not depend merely on electric charge but also on the presence of protonated/deprotonated phenolic groups, together with ester-linked carbonyl oxygen, which seem to be good candidates for a co-ordinate type of calcium-binding. Nanoplast embedding turned out to be the most adequate and fastest preparation for X-ray microanalysis and, apart from retaining calcium, allowed the detection of zinc and copper inside the physodes.

Harvard-Zitierstil: Tretyn, A., Grolig, F., Magdowski, G. and Wagner, G. (1996) Selective binding of Ca2+, Zn2+, Cu2+ and K+ by the physodes of the green alga Mougeotia scalaris, Folia Histochemica et Cytobiologica, 34(2), pp. 103-108

APA-Zitierstil: Tretyn, A., Grolig, F., Magdowski, G., & Wagner, G. (1996). Selective binding of Ca2+, Zn2+, Cu2+ and K+ by the physodes of the green alga Mougeotia scalaris. Folia Histochemica et Cytobiologica. 34(2), 103-108.