Journalartikel

Jahr der Veröffentlichung: 1995

Seiten: 816-823

Zeitschrift: Theoretical and Applied Genetics

Bandnummer: 91

Heftnummer: 5

ISSN: 0040-5752

eISSN: 1432-2242

DOI Link: https://doi.org/10.1007/BF00220965

Verlag: Springer

Abstract: 
Systematic investigations on the occurrence of differential DNA replication in carrot cultures, expressed at the total genome level, were performed. The genome of Daucus carota L. could be characterized by a pattern of repetitive BstNI fragments that was independent of tissue specificity or cultivar differences. Characterization of the genomic DNA of the secondary phloem of carrot roots, in comparison to the DNA of the induced primary cultures at different growth phases, revealed dramatic differences in the copy number of the repetitive fragments. Highly proliferative tissue showed extensive reduction in the proportion of repetitive sequences in the genome in all of the 37 investigated variants. In contrast, during subsequent transition to stationary growth the repetitive fragments re-amplified. The results suggest that the quantitative genome organisation was involved in the regulation of the growth potential of cells. A hypothesis is discussed suggesting a determining influence of the observed differential DNA replication on cell-cycle rates and the cell program of proliferative tissue by structural and positioning effects on DNA loops. To study the causality of somaclonal variation, research on the relationship between physiological genome variability and the induction of heritable changes is recommended.

Harvard-Zitierstil: ARNHOLDTSCHMITT, B. (1995) PHYSIOLOGICAL-ASPECTS OF GENOME VARIABILITY IN TISSUE-CULTURE .2. GROWTH-PHASE DEPENDENT QUANTITATIVE VARIABILITY OF REPETITIVE BSTNI FRAGMENTS OF PRIMARY CULTURES OF DAUCUS-CAROTA L, TAG Theoretical and Applied Genetics, 91(5), pp. 816-823. https://doi.org/10.1007/BF00220965

APA-Zitierstil: ARNHOLDTSCHMITT, B. (1995). PHYSIOLOGICAL-ASPECTS OF GENOME VARIABILITY IN TISSUE-CULTURE .2. GROWTH-PHASE DEPENDENT QUANTITATIVE VARIABILITY OF REPETITIVE BSTNI FRAGMENTS OF PRIMARY CULTURES OF DAUCUS-CAROTA L. TAG Theoretical and Applied Genetics. 91(5), 816-823. https://doi.org/10.1007/BF00220965