Journal article

Publication year: 1995

Pages: 2967-2971

Journal: European Journal of Immunology

Volume number: 25

Issue number: 10

ISSN: 0014-2980

eISSN: 1521-4141

DOI Link: https://doi.org/10.1002/eji.1830251038

Publisher: Wiley

Abstract: 
We have exploited the pore forming activity of listeriolysin, the hemolysin of Listeria monocytogenes, to activate CD8(+) T cells with soluble proteins in vivo and in vitro. Immunization with soluble, hemolytically active listeriolysin induces both cytotoxic CD8(+) T cells and CD4(+) T cells, and the CD8(+) T cells can be propagated with soluble listeriolysin in vitro. Moreover, conventional antigens like ovalbumin mixed together with listeriolysin are also efficiently introduced into the MHC class I pathway in vitro and in vivo. Hence, listeriolysin effectively directs itself and passenger molecules into the intracellular compartment that leads to the cytotoxic T cell response. In this way, we circumvent the bias of CD8(+) T cells to recognize intracellular antigens presented by major histocompatibility complex class I molecules. As cytotoxic CD8(+) T cells are of pivotal importance in eliminating viral and microbial pathogens, the findings reported here could prove to be useful in vaccine development.

Harvard Citation style: DARJI, A., CHAKRABORTY, T., WEHLAND, J. and WEISS, S. (1995) LISTERIOLYSIN GENERATES A ROUTE FOR THE PRESENTATION OF EXOGENOUS ANTIGENS BY MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I, European Journal of Immunology, 25(10), pp. 2967-2971. https://doi.org/10.1002/eji.1830251038

APA Citation style: DARJI, A., CHAKRABORTY, T., WEHLAND, J., & WEISS, S. (1995). LISTERIOLYSIN GENERATES A ROUTE FOR THE PRESENTATION OF EXOGENOUS ANTIGENS BY MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I. European Journal of Immunology. 25(10), 2967-2971. https://doi.org/10.1002/eji.1830251038