Journal article

Publication year: 1995

Pages: 8-14

Journal: Cancer Genetics and Cytogenetics

Volume number: 79

Issue number: 1

ISSN: 0165-4608

DOI Link: https://doi.org/10.1016/0165-4608(94)00096-T

Publisher: Elsevier

Abstract: 
Using fluorescence in situ hybridization (FISH), we studied chromosome 8 abnormalities in 30 children with mature B-cell acute lymphoblastic leukemia (B-ALL) or B-cell non-Hodgkin lymphoma (B-NHL). FISH was performed on metaphase spreads and interphase nuclei with a whole chromosome 8 painting probe. Fifteen patients were studied retrospectively after metaphases from the malignant cell specimen had already been G-banded. When interphase nuclei were examined, FISH was able to confirm t(8;14)(q24;q32) in all nine patients positive by previous G-banding. FISH, however, was positive in metaphase spreads from only seven patients. Another 15 patients were included in a prospective study In six of them (40%), a translocation involving chromosome 8 was shown by a split small segment (8q24-8qter) on interphase nuclei. Analysis of metaphase spreads showed only three positive cases each by FISH or G-banding, respectively, with corresponding results in two patients. By interphase FISH, trisomy of chromosome 8 also was detectable. In three patients shown by G-banding to have trisomy, interphase FISH study showed high scores of three chromosome 8 signal positive cells. There was no cross-hybridization to other chromosomes interfering with FISH analysis. FISH analysis on interphase nuclei using a whole chromosome 8 hybridization probe will supplement and can be more sensitive than metaphase cytogenetic techniques for detection of chromosome 8 rearrangements in B-ALL/NHL.

Harvard Citation style: NISHIDA, K., RITTERBACH, J., REPP, R., HARBOTT, J. and LAMPERT, F. (1995) CHARACTERIZATION OF CHROMOSOME-8 ABNORMALITIES BY FLUORESCENCE IN-SITU HYBRIDIZATION IN CHILDHOOD B-ACUTE LYMPHOBLASTIC-LEUKEMIA NON-HODGKIN-LYMPHOMA, Cancer Genetics and Cytogenetics, 79(1), pp. 8-14. https://doi.org/10.1016/0165-4608(94)00096-T

APA Citation style: NISHIDA, K., RITTERBACH, J., REPP, R., HARBOTT, J., & LAMPERT, F. (1995). CHARACTERIZATION OF CHROMOSOME-8 ABNORMALITIES BY FLUORESCENCE IN-SITU HYBRIDIZATION IN CHILDHOOD B-ACUTE LYMPHOBLASTIC-LEUKEMIA NON-HODGKIN-LYMPHOMA. Cancer Genetics and Cytogenetics. 79(1), 8-14. https://doi.org/10.1016/0165-4608(94)00096-T