Konferenzpaper

Jahr der Veröffentlichung: 1995

Seiten: 481-485

Zeitschrift: Agronomie

Bandnummer: 15

Heftnummer: 7-8

ISSN: 0249-5627

Open Access Status: Green

DOI Link: https://doi.org/10.1051/agro:19950717

Konferenz: VIIth Conference on Virus Diseases of Poaceae in Europe

Verlag: EDP Sciences

Abstract: 
Studies to identify an RAPD marker linked to the BaMMV/BaYMV resistance gene ym4 were carried out on F-1 anther derived doubled haploid (DH) barley lines from a cross between the BaMMV/BaYMV susceptible cultivar 'Igri' and the resistant cultivar 'Franka' (ym4). For initial primer screening, bulked segregant analysis was used. Out of 148 decamer primers screened, only primer OP-Z04 revealed polymorphism resulting in an additional 660 bp band in the susceptible bulk. Linkage analysis carried out on 287 DH lines revealed that OP-Z04H660 is very closely linked to ym4 facilitating efficient marker-based selection for BaMMV/BaYMV resistance encoded by this gene. Additional studies showed that OP-Z04H660 can discriminate perfectly between resistant (ym4) and susceptible commercial barley cultivars.

Harvard-Zitierstil: Ordon, F., Bauer, E., Friedt, W. and Graner, A. (1995) Marker-based selection for the ym4 BaMMV-resistance gene in barley using RAPDs, Agronomie, 15(7-8), pp. 481-485. https://doi.org/10.1051/agro:19950717

APA-Zitierstil: Ordon, F., Bauer, E., Friedt, W., & Graner, A. (1995). Marker-based selection for the ym4 BaMMV-resistance gene in barley using RAPDs. Agronomie. 15(7-8), 481-485. https://doi.org/10.1051/agro:19950717