Journal article

GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR


Authors listMOSGES, G; FRIEDT, W

Publication year1994

Pages114-124

JournalPlant Breeding

Volume number113

Issue number2

ISSN0179-9541

DOI Linkhttps://doi.org/10.1111/j.1439-0523.1994.tb00713.x

PublisherWiley


Abstract
Three different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybrids were analysed by three isozymes, oligonucleotides (GACA)(4), (GATA)(4) and (GGAT)(4) as hybridization probes, and 70 primers for PCR analysis. Polymorphisms were visible with each type of marker. However, the repeated probes, especially (GATA)(4), revealed the highest degree of variability and seem to be most appropriate for the generation of genotype-specific 'fingerprints.'



Citation Styles

Harvard Citation styleMOSGES, G. and FRIEDT, W. (1994) GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR, Plant Breeding, 113(2), pp. 114-124. https://doi.org/10.1111/j.1439-0523.1994.tb00713.x

APA Citation styleMOSGES, G., & FRIEDT, W. (1994). GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR. Plant Breeding. 113(2), 114-124. https://doi.org/10.1111/j.1439-0523.1994.tb00713.x



Keywords


AMPLIFIED POLYMORPHIC DNAAP-PCRARBITRARY PRIMERSDNA FINGERPRINTINGGENOMESHELIANTHUS ANNUUSISOZYMESMARKERSMINISATELLITESRAPD

Last updated on 2025-02-04 at 06:07