Journalartikel

Jahr der Veröffentlichung: 1993

Seiten: 452-462

Zeitschrift: Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

Bandnummer: 163

Heftnummer: 6

ISSN: 0174-1578

Verlag: Springer

Abstract: 
The intracellular distribution of potassium in Malpighian tubules from Drosophila larva was measured by electron probe X-ray microanalysis of freeze-dried cryosections. Application of amiloride alone to the haemolymph space had no effect on the intracellular potassium concentration in the region of intermediate cytoplasm (between the basal region of basal membrane infoldings and the apical brush border), whereas a potassium increase as well as a chloride increase was observed after simultaneous blocking of the potassium conductance of the basal membrane with barium. Injected bafilomycin and amiloride applied in the haemolymph caused an increase of the potassium content in the basal cytoplasm but not in the microvilli. In addition, the intracellular water portion was decreased by bafilomycin. pH measurements in isolated larval anterior tubules with proton-selective microelectrodes showed that bafilomycin added to the bathing solution caused a decrease in intracellular pH. Addition of amiloride had no significant effect on intracellular pH, but the pH of the luminal fluid was decreased within 1 min by 0.5 pH units. The amiloride-induced luminal pH decrease could be inhibited by the metabolic blocker KCN as well as by bafilomycin. Furthermore, removing potassium from the bathing saline caused a slow luminal acidification, which could be blocked by KCN. Our results support the hypothesis of a functionally coupled transport system in the apical membrane consisting of a bafilomycin-sensitive V-ATPase and a K+-dependent, amiloride-sensitive K+/H+ exchange system.

Harvard-Zitierstil: WESSING, A., BERTRAM, G. and ZIEROLD, K. (1993) EFFECTS OF BAFILOMYCIN A1 AND AMILORIDE ON THE APICAL POTASSIUM AND PROTON GRADIENTS IN DROSOPHILA MALPIGHIAN TUBULES STUDIED BY X-RAY-MICROANALYSIS AND MICROELECTRODE MEASUREMENTS, Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology, 163(6), pp. 452-462

APA-Zitierstil: WESSING, A., BERTRAM, G., & ZIEROLD, K. (1993). EFFECTS OF BAFILOMYCIN A1 AND AMILORIDE ON THE APICAL POTASSIUM AND PROTON GRADIENTS IN DROSOPHILA MALPIGHIAN TUBULES STUDIED BY X-RAY-MICROANALYSIS AND MICROELECTRODE MEASUREMENTS. Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. 163(6), 452-462.