Journal article

Publication year: 1993

Pages: 219-224

Journal: Mutation research - Genetic toxicology

Volume number: 299

Issue number: 3-4

ISSN: 0921-8262

DOI Link: https://doi.org/10.1016/0165-1218(93)90098-X

Publisher: Elsevier

Abstract: 
The radiation-sensitive mutant rad 54-3 of Saccharomyces cerevisiae temperature-conditional for the repair of DNA double-strand breaks was exposed to 254 nm ultraviolet radiation and incubated at the restrictive and permissive temperatures. A large difference in survival was seen indicating the involvement of double-strand breaks in cellular inactivation at least in this strain. Pulsed-field gel electrophoresis of DNA showed that double-strand breaks are not directly induced but develop upon incubation under growth conditions. Their number is highest after about 4 h, after 8 h repair is complete in wild-type cells. With the aid of the excision-deficient double mutant rad3rad54 it could be demonstrated that strand break formation proceeds independent of excision repair.

Harvard Citation style: Kiefer, J. and Feige, M. (1993) The significance of DNA double-strand breaks in the UV inactivation of yeast cells, Mutation research - Genetic toxicology, 299(3-4), pp. 219-224. https://doi.org/10.1016/0165-1218(93)90098-X

APA Citation style: Kiefer, J., & Feige, M. (1993). The significance of DNA double-strand breaks in the UV inactivation of yeast cells. Mutation research - Genetic toxicology. 299(3-4), 219-224. https://doi.org/10.1016/0165-1218(93)90098-X