Journal article

Publication year: 1993

Pages: 793-800

Journal: Theoretical and Applied Genetics

Volume number: 85

Issue number: 6-7

ISSN: 0040-5752

DOI Link: https://doi.org/10.1007/BF00225021

Publisher: Springer

Abstract: 
Rapid genomic DNA variation due to methylation and copy number alteration was observed in carrot root explants 6 h after inoculation and during a 36-h period of exponential callus growth. De novo methylation and amplification of restricted BspNI fragments of low molecular weight occurred before cell cycle activation and should, therefore, be independent of progression through the S-phase of the cell cycle. Growth regulators seemed to influence the amplification pattern indirectly by regulating cell division activity. In exponentially growing callus tissue the copy number of most of the repetitive fragments was dramatically reduced. It is presumed that this reduction in the copy number of repetitive fragments is characteristic of 'rejuvenilization'. 3-Indole-acetic-acid (IAA) and inositol in the medium increased the degree of unspecific genomic DNA methylation in growing rhizogenic carrot callus tissue in the absence of kinetin, which inhibits root induction at that stage. A possible relation to the induction of rhizogenesis is considered. The observed reduction in number of repetitive restriction fragments and the increase in DNA methylation are gross changes covering the total genome. The results are discussed in relation to the controversy concerning the general biological significance of the methylation and amplification of DNA sequences.

Harvard Citation style: ARNHOLDTSCHMITT, B. (1993) RAPID CHANGES IN AMPLIFICATION AND METHYLATION PATTERN OF GENOMIC DNA IN CULTURED CARROT ROOT EXPLANTS (DAUCUS-CAROTA L), TAG Theoretical and Applied Genetics, 85(6-7), pp. 793-800. https://doi.org/10.1007/BF00225021

APA Citation style: ARNHOLDTSCHMITT, B. (1993). RAPID CHANGES IN AMPLIFICATION AND METHYLATION PATTERN OF GENOMIC DNA IN CULTURED CARROT ROOT EXPLANTS (DAUCUS-CAROTA L). TAG Theoretical and Applied Genetics. 85(6-7), 793-800. https://doi.org/10.1007/BF00225021