Journal article

Publication year: 1993

Pages: 286-291

Journal: Cytogenetics And Cell Genetics

Volume number: 64

Issue number: 3-4

ISSN: 0301-0171

DOI Link: https://doi.org/10.1159/000133594

Abstract: 
Flow cytometric analysis of enzymatically decondensed, DAPI-stained spermatozoa was performed to confirm the suspected production of unbalanced spermatozoa in heterozygous rams carrying a 1;20 translocation. High-precision flow cytometry (coefficient of variation, 0.6-0.8 %) with a PAS II flow cytometer depicted Y- and X-chromosome-bearing spermatozoa from three cytogenetically normal rams as two distinct peaks. The difference in DNA fluorescence intensity between the gonosomes averaged 4.8%. Analysis of sperm samples from three heterozygous 1;20 translocation carriers yielded histograms with five peak distributions. The individual peaks were attributed to spermatozoa with a normal, balanced, and unbalanced chromosomal status. Peaks within Y- and X-spermatozoa populations were distributed in a ratio of 1:2:1 and were almost completely separated, with a coefficient of variation of 0.5-0.6%. Owing to the relative size of the translocated chromosomal segment (2.4% of the total DNA content, as determined from the flow cytometric data), histograms with five instead of the expected six peaks were observed.

Harvard Citation style: LEWALSKI, H., OTTO, F., KRANERT, T. and WASSMUTH, R. (1993) FLOW CYTOMETRIC DETECTION OF UNBALANCED RAM SPERMATOZOA FROM HETEROZYGOUS 1 20 TRANSLOCATION CARRIERS, Cytogenetics And Cell Genetics, 64(3-4), pp. 286-291. https://doi.org/10.1159/000133594

APA Citation style: LEWALSKI, H., OTTO, F., KRANERT, T., & WASSMUTH, R. (1993). FLOW CYTOMETRIC DETECTION OF UNBALANCED RAM SPERMATOZOA FROM HETEROZYGOUS 1 20 TRANSLOCATION CARRIERS. Cytogenetics And Cell Genetics. 64(3-4), 286-291. https://doi.org/10.1159/000133594