Journal article

Publication year: 1992

Pages: 1515-1518

Journal: Journal of Experimental Botany

Volume number: 43

Issue number: 256

ISSN: 0022-0957

DOI Link: https://doi.org/10.1093/jxb/43.11.1515

Publisher: Oxford University Press

Abstract: 
The effects of externally induced metabolic perturbations are often studied through changes of the enzyme activity patterns in crude plant extracts. From glutamate dehydrogenase (GDH) it is reported that environmental changes not only influence the amount of the enzymatic activity, but also the ratio of the aminating to the deaminating activities (NADH/NAD+ ratio). Using crude cell extracts of suspension cultures of wheat (Triticum aestivum L. cv. Heines Koga II) we find evidence that the pretreatment of the homogenate directly influences this ratio. Dialysis of these crude cell extracts resulted in a 70% loss of the NAD+ activity, while the NADH activity remained unchanged. The deaminating activity in the dialysed extract could be completely restored upon addition of a dialysable factor which was identified to be malate. The interference of malate with the glutamate dehydrogenase reaction is caused through the action of malate dehydrogenase and glutamate oxaloacetate transaminase which are both present in high activities in the extracts. Only in exhaustively dialysed cell extracts can the proper deaminating GDH activity be determined. The results are discussed in the light of the controversial reports on the variable ratio of the NADH/NAD+ activity of GDH.

Harvard Citation style: FRICKE, W. and PAHLICH, E. (1992) MALATE - A POSSIBLE SOURCE OF ERROR IN THE NAD GLUTAMATE-DEHYDROGENASE ASSAY, Journal of Experimental Botany, 43(256), pp. 1515-1518. https://doi.org/10.1093/jxb/43.11.1515

APA Citation style: FRICKE, W., & PAHLICH, E. (1992). MALATE - A POSSIBLE SOURCE OF ERROR IN THE NAD GLUTAMATE-DEHYDROGENASE ASSAY. Journal of Experimental Botany. 43(256), 1515-1518. https://doi.org/10.1093/jxb/43.11.1515