Journal article

Publication year: 1992

Pages: 559-565

Journal: European Journal of Biochemistry

Volume number: 207

Issue number: 2

ISSN: 0014-2956

Open access status: Bronze

DOI Link: https://doi.org/10.1111/j.1432-1033.1992.tb17082.x

Publisher: Wiley: No OnlineOpen

Abstract: 
Methanobacterium wolfei is a thermophilic methanogenic archaeon which requires tungsten or molybdenum for growth. We have found that the organism contains two formylmethanofuran dehydrogenases, one of which is a tungsten enzyme. Indirect evidence indicates that the other formylmethanofuran dehydrogenase is a molybdenum enzyme. The tungsten enzyme was purified and characterized. The native enzyme had an apparent molecular mass of 130 kDa. SDS/PAGE revealed a composition of three subunits of apparent molecular mass 35, 51 and 64 kDa, the N-terminal amino acid sequences of two of which were determined. 0.3-0.4 mol tungsten/mol enzyme was found but no molybdenum. The pterin cofactor was identified as molybdopterin guanine dinucleotide. The purified enzyme exhibited a specific activity of 8.3-mu-mol.min-1.mg protein-1 and an apparent K(m) for formylmethanofuran and methylviologen of 13-mu-M and 0.4 mM, respectively. The optimum temperature for activity was 65-degrees-C. At 40-60-degrees-C, the rate increased with a Q10 of 1.9; the activation energy of the reaction was 45 kJ/mol. The enzyme was found to require potassium ions for thermostability. The oxygen-sensitive enzyme was not inactivated by cyanide.

Harvard Citation style: SCHMITZ, R., RICHTER, M., LINDER, D. and THAUER, R. (1992) A TUNGSTEN-CONTAINING ACTIVE FORMYLMETHANOFURAN DEHYDROGENASE IN THE THERMOPHILIC ARCHAEON METHANOBACTERIUM-WOLFEI, European Journal of Biochemistry, 207(2), pp. 559-565. https://doi.org/10.1111/j.1432-1033.1992.tb17082.x

APA Citation style: SCHMITZ, R., RICHTER, M., LINDER, D., & THAUER, R. (1992). A TUNGSTEN-CONTAINING ACTIVE FORMYLMETHANOFURAN DEHYDROGENASE IN THE THERMOPHILIC ARCHAEON METHANOBACTERIUM-WOLFEI. European Journal of Biochemistry. 207(2), 559-565. https://doi.org/10.1111/j.1432-1033.1992.tb17082.x