Journalartikel

Jahr der Veröffentlichung: 1992

Seiten: 487-492

Zeitschrift: Histochemistry

Bandnummer: 97

Heftnummer: 6

ISSN: 0301-5564

DOI Link: https://doi.org/10.1007/BF00316068

Verlag: Springer

Abstract: 
Effect of the covalently cross-linking agents glutardialdehyde and osmium tetroxide, and of adsorption of the vital dye, neutral red, to the matrix of the calcium-binding "vesicles" from the green alga Mougeotia scalaris has been analysed in situ, both in terms of structural preservation and of the calcium-binding capacity of the vesicles. Upon cell fixation in glutardialdehyde without OsO4, the vesicles appear to dissolve, but upon simultaneous fixation in glutardialdehyde with OsO4 (1 % w/v), the vesicles retain a globular form, are evenly stained by osmium and appear to be surrounded by a membrane-like structure. This structure was also observed around the vesicles in cells preincubated for 10 min in 0.1 mM neutral red and then fixed in glutardialdehyde/OsO4 for 1 h. More detailed information of the matrix structure is obtained when simultaneous fixation of the Mougeotia cells was shortened to 15 min: a membrane-like structure was no longer observed around the vesicles. After cell treatment in the presence of neutral red, no calcium at all was found inside the vesicles. A small amount of calcium remained, when cells were fixed simultaneously and extensively in the absence of neutral red. However, calcium was found, to a considerable extent, inside the vesicles after short simultaneous fixation of the cells in the absence of neutral red. Based on the ultrastructural and elemental features presented here, the calcium-binding vesicles in Mougeotia appear to represent a member of the large family of (calcium-binding) physodes in lower plants (CaBP).

Harvard-Zitierstil: TRETYN, A., GROLIG, F., MAGDOWSKI, G. and WAGNER, G. (1992) ELECTRON-MICROSCOPIC CHARACTERIZATION OF CALCIUM-BINDING PHYSODES IN THE GREEN-ALGA MOUGEOTIA-SCALARIS, Histochemistry, 97(6), pp. 487-492. https://doi.org/10.1007/BF00316068

APA-Zitierstil: TRETYN, A., GROLIG, F., MAGDOWSKI, G., & WAGNER, G. (1992). ELECTRON-MICROSCOPIC CHARACTERIZATION OF CALCIUM-BINDING PHYSODES IN THE GREEN-ALGA MOUGEOTIA-SCALARIS. Histochemistry. 97(6), 487-492. https://doi.org/10.1007/BF00316068