Journal article

Publication year: 1992

Pages: 789-792

Journal: European Journal of Biochemistry

Volume number: 204

Issue number: 2

ISSN: 0014-2956

Open access status: Bronze

DOI Link: https://doi.org/10.1111/j.1432-1033.1992.tb16696.x

Publisher: Wiley: No OnlineOpen

Abstract: 
The location of amino acids with direct influence on toxic activity in the pore-forming cytotoxin from Pseudomonas aeruginosa was tested by site-directed mutagenesis. Mutant fragments obtained by the polymerase-chain reaction were subcloned into a cytotoxin gene-bearing plasmid to minimize the possibility of amplification error. Our data suggest an important role of cysteines for toxic activity of cytotoxin. Furthermore, domain-faciliting-binding to plasma membranes is located on the C-terminal side of Cys215.

Harvard Citation style: XIONG, G. and LUTZ, F. (1992) SITE-DIRECTED MUTAGENESIS OF THE PSEUDOMONAS-AERUGINOSA CYTOTOXIN FOR PROBING TOXIC ACTIVITY, European Journal of Biochemistry, 204(2), pp. 789-792. https://doi.org/10.1111/j.1432-1033.1992.tb16696.x

APA Citation style: XIONG, G., & LUTZ, F. (1992). SITE-DIRECTED MUTAGENESIS OF THE PSEUDOMONAS-AERUGINOSA CYTOTOXIN FOR PROBING TOXIC ACTIVITY. European Journal of Biochemistry. 204(2), 789-792. https://doi.org/10.1111/j.1432-1033.1992.tb16696.x