Conference paper

DETECTION OF ACTIN AND LOCALIZATION OF PHYTOCHROME IN THE GREEN-ALGA MOUGEOTIA BY MONOCLONAL-ANTIBODIES


Authors listHANSTEIN, C; LANGE, U; SCHNEIDERPOETSCH, HAW; GROLIG, F; WAGNER, G

Publication year1992

Pages223-230

JournalActa Histochemica: Research and Methods in Cell and Tissue Biology

ISSN0065-1281

Conference3RD SYMP ON THE CYTOSKELETON : BIOLOGICAL AND MEDICAL ASPECTS

PublisherElsevier


Abstract

In order to get insight into the topological relationship of phytochrome and the actin cytoskeleton in Mougeotia, phytochrome was localized by indirect immunofluorescence in fixed protoplasts of Mougeotia with the monoclonal antibody Z-3B1, raised against purified Zea mays phytochrome (Schneider-Poetsch et al. 1988, Planta 173, 61-72). So far no detection of phytochrome in the immunoblot was possible by this antibody, in contrast to the detection of phytochrome in the immunoblot was possible by this antibody, in contrast to the detection of actin by the monoclonal anti-actin C4 (Lessard 1988, Cell Motil. Cytoskeleton 10, 349-362).

Preliminary results are presented on attempts to enrich plant factors which interfere with the G-/F-actin equilibrium, as probed by the viscometric falling ball assay.




Citation Styles

Harvard Citation styleHANSTEIN, C., LANGE, U., SCHNEIDERPOETSCH, H., GROLIG, F. and WAGNER, G. (1992) DETECTION OF ACTIN AND LOCALIZATION OF PHYTOCHROME IN THE GREEN-ALGA MOUGEOTIA BY MONOCLONAL-ANTIBODIES, Acta Histochemica: Research and Methods in Cell and Tissue Biology, pp. 223-230

APA Citation styleHANSTEIN, C., LANGE, U., SCHNEIDERPOETSCH, H., GROLIG, F., & WAGNER, G. (1992). DETECTION OF ACTIN AND LOCALIZATION OF PHYTOCHROME IN THE GREEN-ALGA MOUGEOTIA BY MONOCLONAL-ANTIBODIES. Acta Histochemica: Research and Methods in Cell and Tissue Biology, 223-230.



Keywords


BINDING PROTEINSCHARACHLOROPLASTSCYTOPLASMMESOTAENIUMMUSCLE ACTIN

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