Journalartikel

Jahr der Veröffentlichung: 1991

Seiten: 422-427

Zeitschrift: Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection

Bandnummer: 98

Heftnummer: 4

ISSN: 0340-8159

Verlag: Ulmer

Abstract: 
Extracts from ground leaves of Melia azedarach L. inhibit growth and disrupt metamorphosis of the fourth instar larvae of the Mexican bean beetle, Epilachna varivestis Muls. A biologically active principle was isolated by open column liquid chromatography (LC), preparative thin layer chromatography (TLC) and preparative high performance liquid chromatography (HPLC). Analytical TLC (chloroform - methanol 19:1) resulted in a single spot with a R(f) value of 0.35. Analytical HPLC on a RP-18 column (Lichrospher 5-mu-m, 250 x 4 mm) with acetonitrile and water (45 % : 55 %) as a mobile phase showed a retention time of the substrance of 5.0 min. UV-scanning revealed two maxima at 210 nm and 280 nm. A 100 % larval mortality was observed with the pure substance below 2 ppm in the bioassay.

Harvard-Zitierstil: ZHU, J. and ERMEL, K. (1991) ISOLATION OF A SUBSTANCE WITH GROWTH-DISTURBING PROPERTIES ON THE MEXICAN BEAN BEETLE EPILACHNA-VARIVESTIS MULS, FROM THE LEAVES OF MELIA-AZEDARACH L, Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection, 98(4), pp. 422-427

APA-Zitierstil: ZHU, J., & ERMEL, K. (1991). ISOLATION OF A SUBSTANCE WITH GROWTH-DISTURBING PROPERTIES ON THE MEXICAN BEAN BEETLE EPILACHNA-VARIVESTIS MULS, FROM THE LEAVES OF MELIA-AZEDARACH L. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz = Journal of plant diseases and protection. 98(4), 422-427.