Journal article

DETECTION OF BOVINE PAPILLOMAVIRUS DNA IN EQUINE SARCOIDS BY POLYMERASE CHAIN-REACTION (PCR)


Authors listTEIFKE, JP; WEISS, E

Publication year1991

Pages185-187

JournalBerliner und Münchener Tierärztliche Wochenschrift

Volume number104

Issue number6

ISSN0005-9366

PublisherSchlütersche Verlagsgesellschaft


Abstract
Unfixed and formalin-fixed frozen sections and paraffin-sections of histopathologically confirmed sarcoids of 20 horses were studied in the PCR. The used set of primers was located in the E5 open reading frame fitting both to bovine papillomavirus 1 (BPV-1) and BPV-2. Independant of the quality of the use tissues BPV-DNA was detected in all 20 sarcoids. By cleaving with restriction endonuclease Bst XI it was shown that the DNA-sequences amplified by PCR were identical with that of BPV-1. The results support the general view that BPV play an important role in equine sarcoids.



Citation Styles

Harvard Citation styleTEIFKE, J. and WEISS, E. (1991) DETECTION OF BOVINE PAPILLOMAVIRUS DNA IN EQUINE SARCOIDS BY POLYMERASE CHAIN-REACTION (PCR), Berliner und Münchener Tierärztliche Wochenschrift, 104(6), pp. 185-187

APA Citation styleTEIFKE, J., & WEISS, E. (1991). DETECTION OF BOVINE PAPILLOMAVIRUS DNA IN EQUINE SARCOIDS BY POLYMERASE CHAIN-REACTION (PCR). Berliner und Münchener Tierärztliche Wochenschrift. 104(6), 185-187.


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