Journal article

Publication year: 1990

Pages: 2659-2663

Journal: Infection and Immunity

Volume number: 58

Issue number: 8

ISSN: 0019-9567

eISSN: 1098-5522

Open access status: Green

DOI Link: https://doi.org/10.1128/IAI.58.8.2659-2663.1990

Publisher: American Society for Microbiology

Abstract: 
Low doses of Escherichia coli hemolysin cause thromboxane-mediated hypertension and vascular leakage in blood-free perfused rabbit lungs (W. Seeger, H. Walter, N. Suttorp, M. Muhyl, and S. Bhakdi, J. Clin. Invest. 84:220-227, 1989). The recirculating buffer medium and bronchoalveolar lavage fluid lungs exposed to heolysin (2.5 hemolytic units per ml) in the presence of cyclooxygenase inhibitor were analyzed for leukotrienes (LTs) and hydroxyeicosatetraenoic acids (HETEs) by reverse-phase and straight-phase high-pressure liquid chromatographic techniques combined with UV spectrum analysis and post-high-pressure liquid chromatography radioimmunoassay. A rapid release of large amounts of cysteine-LTs and leukotrine B4 (LTB4) into the intravascular space was noted (total sum, .apprx. 4 to 5 .mu.g). Similar quantities have hitherto been elicited only by high concentrations of the artificial calcium ionophor A 23187. Moreover, a marked liberation of 5-HETE and 12-hydroxyhepadecatrienoic acid into the buffer medium occurred, whereas LTB4 represented the predominant compound in the lavage fluid. The hemolysin-induced burst of LT and HETE generation preceded the onset of vascular leakage. The outstanding capacity of E. coli hemolysin to produce the liberation of potent lipid mediators is probably relevant to the pathway of vascular injury and amplification of inflammatory events during severe infection with hemolytic E. coli strains.

Harvard Citation style: GRIMMINGER, F., WALMRATH, D., BIRKEMEYER, R., BHAKDI, S. and SEEGER, W. (1990) LEUKOTRIENE AND HYDROXYEICOSATETRAENOIC ACID GENERATION ELICITED BY LOW-DOSES OF ESCHERICHIA-COLI HEMOLYSIN IN RABBIT LUNGS, Infection and Immunity, 58(8), pp. 2659-2663. https://doi.org/10.1128/IAI.58.8.2659-2663.1990

APA Citation style: GRIMMINGER, F., WALMRATH, D., BIRKEMEYER, R., BHAKDI, S., & SEEGER, W. (1990). LEUKOTRIENE AND HYDROXYEICOSATETRAENOIC ACID GENERATION ELICITED BY LOW-DOSES OF ESCHERICHIA-COLI HEMOLYSIN IN RABBIT LUNGS. Infection and Immunity. 58(8), 2659-2663. https://doi.org/10.1128/IAI.58.8.2659-2663.1990