Journal article

Publication year: 1990

Pages: 225-230

Journal: Protoplasma

Volume number: 157

Issue number: 1-3

ISSN: 0033-183X

eISSN: 1615-6102

DOI Link: https://doi.org/10.1007/BF01322655

Publisher: Springer

Abstract: 
F-actin was localized in Mougeotia interphase cells by rhodamine phalloidin (RLP) using an extended, formaldehyde-based fixation protocol, which included a minimal concentration of 0.05% (v/v) glutardialdehyde and stabilization of the calcium-binding vesicles by presaturation with neutral red. Staining revealed a low level of RLP-fluorescence throughout the cytoplasm. An enhanced level of RLP-fluorescence was found around the nucleus and in mostly two parallel fringes along each longitudinal chloroplast edge; also close to the chloroplast edge, quite regularly spaced patches of RLP-fluorescence were seen possibly associated with dictyosomes. The diffuse staining indicates lack of F-actin bundles in Mougeotia filamentous cells, in contrast to Spirogyra interphase cells or Mougeotia protoplasts. The observations upon staining with RLP confirm previous findings by electron microscopy and indicate seemingly single actin filaments throughout the entire Mougeotia filamentous cell. Thus, a special F-actin organization is evident here which for the chloroplast movement is in support of the hypothesis of pigment regulated plasmalemma anchorage sites to actin filaments.

Harvard Citation style: GROLIG, F., WEIGANGKOHLER, K. and WAGNER, G. (1990) DIFFERENT EXTENT OF F-ACTIN BUNDLING IN WALLED CELLS AND IN PROTOPLASTS OF MOUGEOTIA-SCALARIS, Protoplasma, 157(1-3), pp. 225-230. https://doi.org/10.1007/BF01322655

APA Citation style: GROLIG, F., WEIGANGKOHLER, K., & WAGNER, G. (1990). DIFFERENT EXTENT OF F-ACTIN BUNDLING IN WALLED CELLS AND IN PROTOPLASTS OF MOUGEOTIA-SCALARIS. Protoplasma. 157(1-3), 225-230. https://doi.org/10.1007/BF01322655