Journal article

Publication year: 1989

Pages: 159-167

Journal: Journal of Reproductive Immunology

Volume number: 15

Issue number: 2

ISSN: 0165-0378

eISSN: 1872-7603

DOI Link: https://doi.org/10.1016/0165-0378(89)90035-1

Publisher: Elsevier

Abstract: 
To analyse the nature of antibodies which are purported to be essential for the maintenance of normal human pregancy, six centers participated in a workshop of "blind" tests on 19 allosera. Fc-receptor dependent assays detected antibodies with specificity only for HLA. In addition to cytotoxic antibodies, the Fc-receptor dependent immune phagocytosis inhibition test revealed two non-cytotoxic alloantibodies with HLA specificity. These antibodies had high titers and may, therefore, be essentially non-cytotoxic. Murine monoclonal antibodies to HLA-A, B, C or DR (W6/32 and 2MC3) were used to evaluate the methods. These antibodies inhibited immune rosette formation as well as immune phagocytosis. Diluted to concentrations below the threshold of complement-dependent cytotoxicity, the monoclonal antibodies still inhibited the mixed lymphocyte reaction and the immune phagocytosis. A human monoclonal immunnoglobulin M with specificity for monomorphic non-HLA lymphocyte antigens inhibited the mixed lymphocyte reaction. The immune rosette inhibition test exhibited several false positive reactions, e.g. three out for four with a serum that did not contain alloantibodies to blood cells. Non-cytotoxic antibodies were therefore rare in the selected sera of th workshop and they exhibited HLA specificity only. No participant was able to identify pregnancy-maintaining non-HLA-antibodies.

Harvard Citation style: NEPPERT, J., MUELLERECKHARDT, G., NEUMEYER, H., MALCHUS, R., KIEFEL, V., GERHARD, I., et al. (1989) PREGNANCY-MAINTAINING ANTIBODIES - WORKSHOP REPORT (GIESSEN, 1988), Journal of Reproductive Immunology, 15(2), pp. 159-167. https://doi.org/10.1016/0165-0378(89)90035-1

APA Citation style: NEPPERT, J., MUELLERECKHARDT, G., NEUMEYER, H., MALCHUS, R., KIEFEL, V., GERHARD, I., KUHN, U., WESTPHAL, E., & HARPPRECHT, J. (1989). PREGNANCY-MAINTAINING ANTIBODIES - WORKSHOP REPORT (GIESSEN, 1988). Journal of Reproductive Immunology. 15(2), 159-167. https://doi.org/10.1016/0165-0378(89)90035-1