Journalartikel

Jahr der Veröffentlichung: 1989

Seiten: 1677-1685

Zeitschrift: Journal of Virology

Bandnummer: 63

Heftnummer: 4

ISSN: 0022-538X

eISSN: 1098-5514

Open Access Status: Green

DOI Link: https://doi.org/10.1128/JVI.63.4.1677-1685.1989

Verlag: American Society for Microbiology

Abstract: 
The hemagglutinin of influenza (fowl plague) virus was expressed in larvae of Heliothis virescens by using recombinant Autographa californica nuclear polyhedrosis virus (AcNPV) as a vector. Animals were infected with the recombinant virus either by parenteral injection or by feeding. For oral uptake, recombinant virus occluded in polyhedra obtained from cultured Spodoptera frugiperda cells after coinfection with authentic AcNPV was used. Immunohistological analyse of infected animals revealed that the hemagglutinin was expressed only those tissues that are also permissive for the replication of authentic AcNPV. These tissues included hypodermis, fat body, and tracheal matrix. After oral infection, hemagglutinin was also detected in individual gut cells. The amount of hemagglutinin synthesized in larvae after parenteral infection was 0.3% of the total protein, compared with 5% obtained in cultured insect cells. The hemagglutinin was transported to the cell surface and expressed in polarized cells only at the apical plasma membrane. It was processed by posttranslational proteolysis into the cleavage products HA1 and HA2. Oligosaccharides were attached by N-glycosidic linkages and were smaller than those found on hemagglutinin obtained from vertebrate cells. Hemagglutinin from larve expressed receptor binding and cell fusion activities, but quantitation of the hemolytic capacity revealed that it was only about half as active as hemagglutinin from vertebrate or insect cell cultures. Chickens immunized with larval tissues containing hemagglutinin were protected from infection with fowl plague virus. These observations demonstrate that live insects are able to produce a recombinant membrane protein of vertebrate origin in biologically active form.

Harvard-Zitierstil: KURODA, K., GRONER, A., FRESE, K., DRENCKHAHN, D., HAUSER, C., ROTT, R., et al. (1989) SYNTHESIS OF BIOLOGICALLY-ACTIVE INFLUENZA-VIRUS HEMAGGLUTININ IN INSECT LARVAE, Journal of Virology, 63(4), pp. 1677-1685. https://doi.org/10.1128/JVI.63.4.1677-1685.1989

APA-Zitierstil: KURODA, K., GRONER, A., FRESE, K., DRENCKHAHN, D., HAUSER, C., ROTT, R., DOERFLER, W., & KLENK, H. (1989). SYNTHESIS OF BIOLOGICALLY-ACTIVE INFLUENZA-VIRUS HEMAGGLUTININ IN INSECT LARVAE. Journal of Virology. 63(4), 1677-1685. https://doi.org/10.1128/JVI.63.4.1677-1685.1989