Journal article

Publication year: 1989

Pages: 1569-1571

Journal: Phytochemistry

Volume number: 28

Issue number: 6

ISSN: 0031-9422

eISSN: 1873-3700

DOI Link: https://doi.org/10.1016/S0031-9422(00)97801-4

Publisher: Elsevier

Abstract: 
Polyethylene glycol (PEG) is often used to produce water stress in plant cell suspension cultures. The amount of soluble protein extracted from potato cell suspension cultures treated with PEG-6000 (10% w/v) decreased to 50%. In addition, the activities of glucose-6-P dehydrogenase, glutamate dehydrogenase and pyrroline-5-carboxylate reductase in these extracts were differentially reduced. High concentrations of PEG (> 5%) in the enzyme assay systems were required to significantly affect the activities of the three enzymes extracted from PEG untreated cells. PEG must be removed from cells before proteins are extracted or enzymes assayed. A rapid procedure to harvest cells and subsequently to remove PEG from cells stressed with this compound is described.

Harvard Citation style: CORCUERA, L., HINTZ, M. and PAHLICH, E. (1989) EFFECT OF POLYETHYLENE-GLYCOL ON PROTEIN EXTRACTION AND ENZYME-ACTIVITIES IN POTATO CELL-CULTURES, Phytochemistry, 28(6), pp. 1569-1571. https://doi.org/10.1016/S0031-9422(00)97801-4

APA Citation style: CORCUERA, L., HINTZ, M., & PAHLICH, E. (1989). EFFECT OF POLYETHYLENE-GLYCOL ON PROTEIN EXTRACTION AND ENZYME-ACTIVITIES IN POTATO CELL-CULTURES. Phytochemistry. 28(6), 1569-1571. https://doi.org/10.1016/S0031-9422(00)97801-4