Journalartikel

Jahr der Veröffentlichung: 1989

Seiten: 419-430

Zeitschrift: Toxicon: An Interdisciplinary Journal on the Toxins Derived from Animals, Plants and Microorganisms

Bandnummer: 27

Heftnummer: 4

ISSN: 0041-0101

eISSN: 1879-3150

DOI Link: https://doi.org/10.1016/0041-0101(89)90204-3

Verlag: Elsevier

Abstract: 
Erythrocytes from four mammalian species were compared with regard to K+loss triggered by palytoxin, to Na+, K+-ATPase activity, and to ouabain sensitivity of both events. Palytoxin sensitivity (EC50) decreased in the order rat, man (.apprxeq. 1 pM) > cattle (.apprxeq. 500 pM) > dog (> 10 nM). Na+, K+-ATPase activity, as measured by Rb uptake, was in the series rat > man > cattle > dog. The glycoside potently inhibited both palytoxin action and ATPase activity in man, cattle and dog erythrocytes, but weakly in those from rats. Ca2+ promoted the palytoxin effects on all erythrocytes. As shown for human erythrocytes, Sr2+ and Ba2+ but not Mg2+ can substitute for Ca2+, and sucrose can substitute for sodium chloride. Human HeLa and bovine adrenomedullary cells also lost their K+ within a few min when exposed to palytoxin (1-10 pM). Ouabain acted as a palytoxin antagonist on both cell types. We conclude that: (a) the ouabain binding site of Na+, K+-ATPase is part of the palytoxin receptor in every cell type tested, (b) high palytoxin sensitivity is not necessarily accompanied by high ouabain sensitivity, and (c) active ion transport is not a precondition for the action of palytoxin or for its inhibition by ouabin.

Harvard-Zitierstil: HABERMANN, E., HUDEL, M. and DAUZENROTH, M. (1989) PALYTOXIN PROMOTES POTASSIUM OUTFLOW FROM ERYTHROCYTES, HELA AND BOVINE ADRENOMEDULLARY CELLS THROUGH ITS INTERACTION WITH NA+, K+-ATPASE, Toxicon: An Interdisciplinary Journal on the Toxins Derived from Animals, Plants and Microorganisms, 27(4), pp. 419-430. https://doi.org/10.1016/0041-0101(89)90204-3

APA-Zitierstil: HABERMANN, E., HUDEL, M., & DAUZENROTH, M. (1989). PALYTOXIN PROMOTES POTASSIUM OUTFLOW FROM ERYTHROCYTES, HELA AND BOVINE ADRENOMEDULLARY CELLS THROUGH ITS INTERACTION WITH NA+, K+-ATPASE. Toxicon: An Interdisciplinary Journal on the Toxins Derived from Animals, Plants and Microorganisms. 27(4), 419-430. https://doi.org/10.1016/0041-0101(89)90204-3