Journalartikel

Jahr der Veröffentlichung: 1988

Seiten: 1-6

Zeitschrift: European Journal of Cell Biology

Bandnummer: 47

Heftnummer: 1

ISSN: 0171-9335

eISSN: 1618-1298

Verlag: Elsevier

Abstract: 
Using electron probe X-ray microanalysis after cryofixation, cryosectioning and freeze-drying we investigated the content of electron-dark vacuoles in the intermediate cell region of the proximal segment of Malpighian tubules in Drosophila larvae. According to this method these vacuoles store sodium and manesium in a high correlation (r = 0.98) of 5:1 ratio. Phosphorus, potassium and sulfur are also stored. In the intermediate groundplasm surrounding the vacuoles the element content is different from that in the vacuoles. The significance of vacuolar sodium and magnesium storge for the ionic metabolism is unknown. In addition to Na, Mg, P, K and S the vacuoles also contain 3-OH-kynurenine and other fluorochromes. With the pyroantimonate technique intravacuolar precipitates were demonstrated. X-ray microanalyhsis of the precipitates revealed sodium and calcium, although following cryofixation calcium was not detectable in the vacuoles by X-ray analysis.

Harvard-Zitierstil: WESSING, A., ZIEROLD, K. and SCHAFER, D. (1988) INTRACELLULAR STORAGE OF SODIUM AND MAGNESIUM IN DROSOPHILA MALPIGHIAN TUBULES - X-RAY-MICROANALYSIS OF NATIVE CRYOSECTIONS, European Journal of Cell Biology, 47(1), pp. 1-6

APA-Zitierstil: WESSING, A., ZIEROLD, K., & SCHAFER, D. (1988). INTRACELLULAR STORAGE OF SODIUM AND MAGNESIUM IN DROSOPHILA MALPIGHIAN TUBULES - X-RAY-MICROANALYSIS OF NATIVE CRYOSECTIONS. European Journal of Cell Biology. 47(1), 1-6.